{"_buckets": {"deposit": "6efd7c84-ad80-4902-a20e-c292a87829c2"}, "_deposit": {"created_by": 5, "id": "12765", "owners": [5], "pid": {"revision_id": 0, "type": "depid", "value": "12765"}, "status": "published"}, "_oai": {"id": "oai:gifu-pu.repo.nii.ac.jp:00012765", "sets": ["200"]}, "author_link": ["17780", "17784", "17783", "17781", "17786", "17782", "17788", "17787", "17785"], "item_1_biblio_info_14": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-06-30", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "45", "bibliographicPageStart": "35", "bibliographicVolumeNumber": "53", "bibliographic_titles": [{"bibliographic_title": "岐阜藥科大學紀要"}, {"bibliographic_title": "The annual proceedings of Gifu College of Pharmacy", "bibliographic_titleLang": "en"}]}]}, "item_1_creator_6": {"attribute_name": "著者名(日)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "永野, 恵司"}], "nameIdentifiers": [{"nameIdentifier": "17780", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "横山, 慎一郎"}], "nameIdentifiers": [{"nameIdentifier": "17781", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "森, 裕志"}], "nameIdentifiers": [{"nameIdentifier": "17782", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_7": {"attribute_name": "著者名よみ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "ナガノ, ケイジ"}], "nameIdentifiers": [{"nameIdentifier": "17783", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ヨコヤマ, シンイチロウ"}], "nameIdentifiers": [{"nameIdentifier": "17784", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "モリ, ヒロシ"}], "nameIdentifiers": [{"nameIdentifier": "17785", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_8": {"attribute_name": "著者名(英)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "NAGANO, Keiji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "17786", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yokoyama, Shin-ichiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "17787", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori, Hiroshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "17788", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_description_1": {"attribute_name": "ページ属性", "attribute_value_mlt": [{"subitem_description": "P(論文)", "subitem_description_type": "Other"}]}, "item_1_description_11": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "腸管粘膜感染菌である腸管出血性大腸菌(enterohemorrhagic Escherichia coli、EHEC)O157:H7の経口感染マウスを用い、粘膜免疫応答の誘導機構の解析を行った。特異抗体は、EHEC O157:H7菌体(Whole cell)を抗原とした酵素免疫測定法(Filtration ELISA)を用い、菌体表面に対する抗体価として評価した。EHEC野生株をICRマウスに経胃接種した場合、本菌は盲腸上皮細胞に接着して4週間以上定着し、接種4週後の糞便中にEHEC菌体に対するIgA抗体の著明な上昇がみられた。一方、EHECの上皮細胞への接着に関わる因子の遺伝子を変異させた菌株はマウス腸管への定着性を示さず、糞便中IgA抗体の誘導もみられなかった。変異株は繰り返し接種しても血中抗体は誘導されるものの糞便中IgA抗体は誘導されなかった。さらに、マウスを抗生物質処置することによって変異株を腸管内に長期定着させた場合も、糞便中IgA抗体は誘導されなかった。以上のように、EHECの上皮細胞への接着は全身性免疫応答の誘導には必ずしも必要ではないが、粘膜免疫応答の誘導には必須であることが示された。", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "The induction mechanism of mucosal immune responses was examined in mice infected orally with an intestinal pathogen, enterohemorrhagic Escherichia coli (EHEC) O157:H7. Antibodies were evaluated as those specific to EHEC O157:H7 cell surface by enzyme linked-immunosorbent assay using EHEC O157:H7 whole cells as an antigen (Filtration ELISA). When the EHEC wild type was inoculated intragastlically into ICR mice, the bacteria adhered on the cecal epithelia and colonized over four weeks, and fecal IgA antibody to the EHEC whole cells markedly increased at four weeks after the inoculation. On the other hand, mutants deleted adherent factors of the EHEC did not colonize in the intestine and did not induce fecal IgA antibody. When the mutant was inoculated repeatedly, fecal IgA antibody was not induced although the serum antibodies were induced. Furthermore, the fecal IgA antibody was not induced even when the mutants resided persistently in the intestine of mice by treatment with an antibiotic. Thus, the adhesion of EHEC to epithelial cells was indispensable to induce the mucosal immune responses but not systemic immune responses.", "subitem_description_type": "Other"}]}, "item_1_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00053514", "subitem_source_identifier_type": "NCID"}]}, "item_1_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Microbiology, School of Dentistry, Aichi-Gakuin University"}, {"subitem_text_language": "en", "subitem_text_value": "Laboratory of MicrobiologH, Gifu Pharmaceutical Universty"}, {"subitem_text_language": "en", "subitem_text_value": "Laboratory of MicrobiologH, Gifu Pharmaceutical Universty"}]}, "item_1_text_2": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_text_value": "総説"}]}, "item_1_text_3": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Review"}]}, "item_1_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "愛知学院大学歯学部微生物学教室"}, {"subitem_text_value": "岐阜薬科大学微生物学教室"}, {"subitem_text_value": "岐阜薬科大学微生物学教室"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2004-06-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00004100995.pdf", "filesize": [{"value": "1.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1300000.0, "url": {"label": "KJ00004100995", "url": "https://gifu-pu.repo.nii.ac.jp/record/12765/files/KJ00004100995.pdf"}, "version_id": "0bee96ca-e523-436f-be19-38bd2b454194"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "粘膜免疫応答", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IgA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "腸管出血性大腸菌", "subitem_subject_scheme": "Other"}, {"subitem_subject": "接着", "subitem_subject_scheme": "Other"}, {"subitem_subject": "マウス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "研究総説", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mucosal immune response", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IgA", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "enterohemorrhagic Escherichia coli", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "adhesion", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mice", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "research review", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "腸管出血性大腸菌経口感染マウスに誘導される腸管内特異免疫応答", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "腸管出血性大腸菌経口感染マウスに誘導される腸管内特異免疫応答"}, {"subitem_title": "Intestinal immune responses in mice infected orally with enterohemorrhagic Escherichia coli", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "5", "path": ["200"], "permalink_uri": "https://gifu-pu.repo.nii.ac.jp/records/12765", "pubdate": {"attribute_name": "公開日", "attribute_value": "2004-06-30"}, "publish_date": "2004-06-30", "publish_status": "0", "recid": "12765", "relation": {}, "relation_version_is_last": true, "title": ["腸管出血性大腸菌経口感染マウスに誘導される腸管内特異免疫応答"], "weko_shared_id": 5}