{"created":"2023-06-19T07:25:17.524884+00:00","id":12968,"links":{},"metadata":{"_buckets":{"deposit":"24aef8e4-a215-4fc2-bd21-f1bed8e7317e"},"_deposit":{"created_by":4,"id":"12968","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"12968"},"status":"published"},"_oai":{"id":"oai:gifu-pu.repo.nii.ac.jp:00012968","sets":["147:219"]},"author_link":["18092","18095","18091","18090","18094","18099","18100","18096","18098","18101","18097","18093"],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-06-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"74","bibliographicPageStart":"68","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"岐阜薬科大学紀要　"},{"bibliographic_title":"The annual proceedings of Gifu Pharmaceutical University","bibliographic_titleLang":"en"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"横山, 雄一"}],"nameIdentifiers":[{"nameIdentifier":"18090","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"井口, 和弘"}],"nameIdentifiers":[{"nameIdentifier":"18091","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"臼井, 茂之"}],"nameIdentifiers":[{"nameIdentifier":"18092","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平野, 和行"}],"nameIdentifiers":[{"nameIdentifier":"18093","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_7":{"attribute_name":"著者名よみ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ヨコヤマ, ユウイチ"}],"nameIdentifiers":[{"nameIdentifier":"18094","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"イグチ, カズヒロ"}],"nameIdentifiers":[{"nameIdentifier":"18095","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ウスイ, シゲユキ"}],"nameIdentifiers":[{"nameIdentifier":"18096","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ヒラノ, カズユキ"}],"nameIdentifiers":[{"nameIdentifier":"18097","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YOKOYAMA, Yuichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18098","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"IGUCHI, Kazuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18099","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"USUI, Shigeyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18100","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HIRANO, Kazuyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18101","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"グリセロールは肝臓における糖新生や脂質合成の材料であるため、肝臓へのグリセロール流入量の変化は様々な代\n謝経路に変調をきたす。アクアポリン9（AQP9）は、主に肝臓において発現が見られ、水分子のみならず、グリセロー\nルや尿素などの低分子溶質をも透過させるチャネル型膜蛋白質である。AMP-activated protein kinase（AMPK）は生体内の\nエネルギーセンサーであり、糖・脂質代謝の恒常性維持に働くセリン/スレオニンキナーゼである。本研究では、AMPK\nの活性化剤である、5-aminoimidazole-4-carboxamide-1--D-ribonucleoside（AICAR）を、ヒト肝癌由来HepG2 細胞に作用さ\nせたところ、AQP9 mRNA の発現量が顕著に減少することを確認した。レポータージーンアッセイや転写因子forkhead box\na2（Foxa2）遺伝子をノックダウンさせた実験の結果から、Foxa2 は、AICAR によるAQP9 遺伝子発現抑制に関わる重要\nな転写調節因子であることを見出した。AICAR により活性化されたAMPK は、Akt のThr-308 残基とSer-473 残基のリン\n酸化を促し、それに伴いFoxa2 がリン酸化されて核内から核外へと移行することを明らかにした。したがって、肝臓での\nグリセロール輸送の観点からAMPK によるコントロールのもとに、AQP9 は肝臓へのグリセロールの流入量を変化させ、\n糖・脂質代謝調節に寄与している可能性が示唆された。","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Change in glycerol influx into the liver causes metabolic alternations in many pathways since glycerol is a substrate for\ngluconeogenesis and lipogenesis in the liver. Aquaporin 9 (AQP9), a channel membrane protein, is expressed mainly in the liver and\npermeable to water and small molecular weight solutes such as glycerol and urea. AMP-activated protein kinase, AMPK, functioning\nas a serine/threonine kinase acts as an energy sensor in the homeostasis of glyco- and lipid-metabolism. In this study, we found that\n5-aminoimidazole-4-carboxamide-1--D-ribonucleoside (AICAR), an AMPK activator, significantly down-regulated AQP9 mRNA\nexpression in human hepatoma HepG2 cells. Forkhead box a2 (Foxa2) was demonstrated to be one of the transcriptional regulators\nof AQP9 gene expression repressed by AICAR from the results of a reporter gene assay and knock-down of the Foxa2 gene by\nsiRNA. Activation of AMPK by AICAR promoted the phosphorylation of Akt at Thr-308 and Ser-473 residues and subsequently\nphosphorylated and excluded Foxa2 from the nucleus. These results suggest the possibility that AQP9 is under AMPK control in the\ninflux of glycerol into the liver and that AQP9 contributes to the glycol- and lipid-metabolism of glycerol transport in the liver.","subitem_description_type":"Other"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA1258935X","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Laboratory of Pharmaceutics, Gifu Pharmaceutical University"},{"subitem_text_language":"en","subitem_text_value":"Department of Pharmacy, Nagoya University Hospital"}]},"item_1_text_2":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_text_value":"総説"}]},"item_1_text_3":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Review"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"岐阜薬科大学医療薬剤学大講座薬剤学研究室"},{"subitem_text_value":"名古屋大学医学部附属病院薬剤部"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-08-04"}],"displaytype":"detail","filename":"AMPK を介した糖・脂質代謝調節に関する研究.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"AMPK を介した糖・脂質代謝調節に関する研究","url":"https://gifu-pu.repo.nii.ac.jp/record/12968/files/AMPK を介した糖・脂質代謝調節に関する研究.pdf"},"version_id":"a30212f0-553e-4d7d-bc5a-50df2f43c961"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AMP-activated protein kinase(AMPK)","subitem_subject_scheme":"Other"},{"subitem_subject":"アクアポリン9(AQP9)","subitem_subject_scheme":"Other"},{"subitem_subject":"forkhead box a2(Foxa2)","subitem_subject_scheme":"Other"},{"subitem_subject":"Akt/PKB","subitem_subject_scheme":"Other"},{"subitem_subject":"核外輸送","subitem_subject_scheme":"Other"},{"subitem_subject":"AMP-activated protein kinase (AMPK)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Aquaporin 9 (AQP9)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"forkhead box a2 (Foxa2)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Akt/PKB","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nuclear exclusion","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"AMPK を介した糖・脂質代謝調節に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"AMPK を介した糖・脂質代謝調節に関する研究"},{"subitem_title":"Regulation of Glucose and Lipid Metabolism via AMPK","subitem_title_language":"en"}]},"item_type_id":"1","owner":"4","path":["219"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-08-04"},"publish_date":"2014-08-04","publish_status":"0","recid":"12968","relation_version_is_last":true,"title":["AMPK を介した糖・脂質代謝調節に関する研究"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-06-19T09:01:29.735581+00:00"}