{"created":"2023-06-19T07:25:24.915372+00:00","id":13204,"links":{},"metadata":{"_buckets":{"deposit":"b8849cc3-c41c-443e-aa65-b57ad8c8e54c"},"_deposit":{"created_by":43,"id":"13204","owners":[43],"pid":{"revision_id":0,"type":"depid","value":"13204"},"status":"published"},"_oai":{"id":"oai:gifu-pu.repo.nii.ac.jp:00013204","sets":["212:265:270"]},"author_link":["19028","19027","19029","19030","19031"],"item_3_biblio_info_3":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"315","bibliographicPageStart":"306","bibliographicVolumeNumber":"51","bibliographic_titles":[{},{"bibliographic_title":"Free radical research","bibliographic_titleLang":"en"}]}]},"item_3_text_4":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_text_value":"10.1080/10715762.2017.1309527"}]},"item_3_textarea_2":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_textarea_value":"There is growing evidence that plasma-activated medium (PAM), which is prepared by non-thermal plasma (NTP) irradiation of cell-free medium, is a beneficial tool for cancer therapy. PAM has been reported to preferentially kill cancer cells; however, its mechanism is not fully understood. Since PAM contains reactive oxygen species (ROS) and reactive nitrogen species, the anti-cancer effects of PAM are thought to be attributed to oxidative stress induced by these reactive molecules. Oxidative stress has been shown to release zinc (Zn2+) from intracellular Zn2+ stores and provoke Zn2+-dependent cell death. We have previously demonstrated that intracellular free Zn2+ plays a critical role in PAM-induced cell death in human neuroblastoma SH-SY5Y cells. In this study, we found that normal human fibroblasts were less susceptible to PAM cytotoxicity compared with SH-SY5Y cells. PAM decreased intracellular NAD+ levels in both cells, whereas the depletion of ATP and mitochondrial ROS generation was hardly observed in fibroblasts. Intracellular mobile Zn2+ contents of fibroblasts were lower than those of SH-SY5Y cells. PAM suppressed the activity of aconitase, which is a tricarboxylic acid cycle enzyme, only in SH-SY5Y cells, and N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), a Zn2+ chelator, counteracted the suppression. The combination treatment with PAM and Zn2+ augmented PAM-induced ATP depletion, mitochondrial ROS generation, and cytotoxicity in fibroblasts. These findings suggest the possibility that cells with high intracellular mobile Zn2+ are susceptible to PAM cytotoxicity. Therefore, we concluded that the differences in mobile Zn2+ levels affect PAM-induced cellular responses."}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Differences in intracellular mobile zinc levels affect susceptibility to plasma-activated medium-induced cytotoxicity.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Differences in intracellular mobile zinc levels affect susceptibility to plasma-activated medium-induced cytotoxicity."}]},"item_type_id":"3","owner":"43","path":["270"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-14"},"publish_date":"2018-06-14","publish_status":"0","recid":"13204","relation_version_is_last":true,"title":["Differences in intracellular mobile zinc levels affect susceptibility to plasma-activated medium-induced cytotoxicity."],"weko_creator_id":"43","weko_shared_id":-1},"updated":"2023-06-19T08:58:10.591156+00:00"}