{"created":"2023-06-19T07:25:24.956653+00:00","id":13205,"links":{},"metadata":{"_buckets":{"deposit":"3248cf99-d316-41b1-8a18-9c752f99ff80"},"_deposit":{"created_by":43,"id":"13205","owners":[43],"pid":{"revision_id":0,"type":"depid","value":"13205"},"status":"published"},"_oai":{"id":"oai:gifu-pu.repo.nii.ac.jp:00013205","sets":["212:265:270"]},"author_link":["19032","19034","19036","19035","19033"],"item_3_biblio_info_3":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11-12","bibliographicPageEnd":"985","bibliographicPageStart":"978","bibliographicVolumeNumber":"51","bibliographic_titles":[{},{"bibliographic_title":"Free radical research","bibliographic_titleLang":"en"}]}]},"item_3_text_4":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_text_value":"10.1080/10715762.2017.1400666"}]},"item_3_textarea_2":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_textarea_value":"Previous reports have demonstrated that excess zinc (Zn2+) released from nerve terminals following cerebral ischemia causes brain injury. Therefore, the disturbance of Zn2+ homeostasis in the brain is thought to be closely linked to neurotoxicity. Recently, hydrogen sulfide (H2S), a gaseous mediator, has been reported to ameliorate ischemic brain injury. However, its mechanism is not fully understood. In this study, we examined whether sodium hydrogen sulfide (NaHS), an H2S donor, protects against Zn2+ cytotoxicity using human neuroblastoma SH-SY5Y cells. NaHS dose-dependently prevented cell death caused by Zn2+ exposure. Treatment of cells with NaHS just before Zn2+ exposure exerted the most potent protection. Zn2+ induced loss of intracellular NAD+ and ATP and mitochondrial dysfunctions, resulting in cytotoxicity associated with failure of energy production; however, NaHS prevented these Zn2+-induced events. In addition, NaHS suppressed Zn2+-dependent activation of metal-responsive transcription factor-1 and induction of metallothionein gene expression. Zn2+ imaging with the Zn2+-specific fluorescent indicator FluoZin-3 revealed that NaHS abolished the elevation of intracellular Zn2+ levels after Zn2+ exposure. These results suggest that entry of Zn2+ into cells was suppressed by NaHS. The measurement of H2S derived from NaHS by o-fluorinated-azido-capped rhodamine (Rho-N3F2), a reaction-based H2S probe, revealed that H2S levels in aqueous solutions were markedly reduced in the presence of Zn2+. This finding suggests the possibility that H2S reacts directly with Zn2+ and decreases extracellular Zn2+ levels. Taken together, we conclude that the protection of NaHS against Zn2+ cytotoxicity is exerted by inhibiting entry of Zn2+ into SH-SY5Ycells."}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hydrogen sulfide ameliorates zinc-induced cell death in neuroblastoma SH-SY5Y cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hydrogen sulfide ameliorates zinc-induced cell death in neuroblastoma SH-SY5Y cells."}]},"item_type_id":"3","owner":"43","path":["270"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-14"},"publish_date":"2018-06-14","publish_status":"0","recid":"13205","relation_version_is_last":true,"title":["Hydrogen sulfide ameliorates zinc-induced cell death in neuroblastoma SH-SY5Y cells."],"weko_creator_id":"43","weko_shared_id":-1},"updated":"2023-06-19T08:58:10.091291+00:00"}