{"created":"2023-06-19T07:25:32.972303+00:00","id":13439,"links":{},"metadata":{"_buckets":{"deposit":"30f8f927-3a80-46a7-a5ad-1eb83d94b86a"},"_deposit":{"created_by":59,"id":"13439","owners":[59],"pid":{"revision_id":0,"type":"depid","value":"13439"},"status":"published"},"_oai":{"id":"oai:gifu-pu.repo.nii.ac.jp:00013439","sets":["212:264:300"]},"author_link":["21594","21592","21595","21596","21593","21588","21591","21589","21590"],"item_3_biblio_info_3":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"184","bibliographicPageStart":"177","bibliographicVolumeNumber":"92","bibliographic_titles":[{},{"bibliographic_title":"Molecular and cellular neurosciences","bibliographic_titleLang":"en"}]}]},"item_3_text_4":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_text_value":"10.1016/j.mcn.2018.09.001 "}]},"item_3_textarea_2":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_textarea_value":"Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized\nby progressive muscle weakness, paralysis, and death. Although its neuropathology\nis well investigated, currently, effective treatments are unavailable. The\nmechanism of ALS involves the aggregation and accumulation of several mutant\nproteins, including mutant copper‑zinc superoxide dismutase (SOD1), TAR DNA\nbinding protein 43 kDa (TDP-43) and fused in sarcoma (FUS) proteins. Previous\nreports have shown that excessive oxidative stress, associated with mitochondrial\ndysfunction and mutant protein accumulation, contributes to ALS pathology. The\npresent study focuses on the promotion of SOD1 misfolding and aggregation by\noxidative stress. Having recently synthesized novel organic gem-dihydroperoxides \n(DHPs) with high anti-oxidant activity, we now examined whether DHPs reduce the\nmutant SOD1-induced intracellular aggregates involved in oxidative stress. We\nfound that, among DHPs, 12AC2O significantly inhibited mutant SOD1-induced cell\ndeath and reduced the intracellular mutant SOD1 aggregates. Moreover,\nimmunofluorescence staining with redox-sensitive dyes showed that 12AC2O reduced \nthe excessive level of intracellular mutant SOD1-induced reactive oxygen species \n(ROS). Additionally, ESR analysis showed that 12AC2O exerts a direct scavenging\neffect against the hydroxyl radical (OH) and the superoxide anion (O2-). These\nresults suggest that 12AC2O is a very useful agent in combination with other\nagents against ALS.\n"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of gem-dihydroperoxides against mutant copper‑zinc superoxide dismutase-mediated neurotoxicity.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of gem-dihydroperoxides against mutant copper‑zinc superoxide dismutase-mediated neurotoxicity."},{"subitem_title":"Effects of gem-dihydroperoxides against mutant copper‑zinc superoxide dismutase-mediated neurotoxicity.","subitem_title_language":"en"}]},"item_type_id":"3","owner":"59","path":["300"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-04"},"publish_date":"2019-03-04","publish_status":"0","recid":"13439","relation_version_is_last":true,"title":["Effects of gem-dihydroperoxides against mutant copper‑zinc superoxide dismutase-mediated neurotoxicity."],"weko_creator_id":"59","weko_shared_id":-1},"updated":"2023-06-19T08:48:48.559912+00:00"}