{"created":"2023-06-19T07:25:33.659566+00:00","id":13457,"links":{},"metadata":{"_buckets":{"deposit":"52e0e721-0f5b-4640-95f1-0fadd9913ff1"},"_deposit":{"created_by":61,"id":"13457","owners":[61],"pid":{"revision_id":0,"type":"depid","value":"13457"},"status":"published"},"_oai":{"id":"oai:gifu-pu.repo.nii.ac.jp:00013457","sets":["212:259","212:259:315"]},"author_link":["21805","21803","21802","21804","21807","21809","21811","21808","21810","21806","21801"],"item_10003_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"917","bibliographicPageStart":"904","bibliographicVolumeNumber":"108","bibliographic_titles":[{},{"bibliographic_title":"Free Radical Biology and Medicine","bibliographic_titleLang":"en"}]}]},"item_10003_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Non-thermal plasma (NTP) is a potential new therapeutic modality for cancer. However, its mechanism of action remains unclear. Herein, we studied the effect of NTP on mesothelioma cells and fibroblasts to understand its anti-proliferative efficacy. Interestingly, NTP demonstrated greater selective anti-proliferative activity against mesothelioma cells relative to fibroblasts than cisplatin, which is used for mesothelioma treatment. The anti-proliferative effect of NTP was enhanced by pre-incubation with the cellular iron donor, ferric ammonium citrate (FAC), and inhibited by iron chelation using desferrioxamine (DFO). Three oxidative stress probes (CM-H2DCFDA, MitoSOX and C11-BODIPY) demonstrated reactive oxygen species (ROS) generation by NTP, which was inhibited by DFO. Moreover, NTP decreased transferrin receptor-1 and increased ferritin-H and -L chain expression that was correlated with decreased iron-regulatory protein expression and RNA-binding activity. This regulation was potentially due to increased intracellular iron in lysosomes, which was demonstrated via the Fe(II)-selective probe, HMRhoNox-M, and was consistent with autophagic-induction. Immunofluorescence using LysoTracker and Pepstatin A probes demonstrated increased cellular lysosome content, which was confirmed by elevated LAMP1 expression. The enhanced lysosomal biogenesis after NTP could be due to the observed increase in fluid-phase endocytosis and early endosome formation. These results suggest NTP acts as a stressor, which results in increased endocytosis, lysosome content and autophagy. In fact, NTP rapidly increased autophagosome formation, as judged by increased LC3B-II expression, which co-localized with LAMP1, indicating autophagolysosome formation. Autophagic-induction by NTP was confirmed using electron microscopy. In summary, NTP acts as a cellular stressor to rapidly induce fluid-phase endocytosis, lysosome biogenesis and autophagy.","subitem_description_type":"Abstract"}]},"item_10003_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.freeradbiomed.2017.04.368","subitem_relation_type_select":"DOI"}}]},"item_10003_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"08915849","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shi, Lei"}],"nameIdentifiers":[{"nameIdentifier":"21801","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Fumiya"}],"nameIdentifiers":[{"nameIdentifier":"21802","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wang, Yue"}],"nameIdentifiers":[{"nameIdentifier":"21803","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okazaki, Yasumasa"}],"nameIdentifiers":[{"nameIdentifier":"21804","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Hiromasa"}],"nameIdentifiers":[{"nameIdentifier":"21805","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizuno, Masaaki"}],"nameIdentifiers":[{"nameIdentifier":"21806","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hori, Masaru"}],"nameIdentifiers":[{"nameIdentifier":"21807","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirayama, Tasuku"}],"nameIdentifiers":[{"nameIdentifier":"21808","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagasawa, Hideko"}],"nameIdentifiers":[{"nameIdentifier":"21809","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Richardson, Des R."}],"nameIdentifiers":[{"nameIdentifier":"21810","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Toyokuni, Shinya"}],"nameIdentifiers":[{"nameIdentifier":"21811","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy","subitem_title_language":"en"}]},"item_type_id":"10003","owner":"61","path":["259","315"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-04"},"publish_date":"2019-03-04","publish_status":"0","recid":"13457","relation_version_is_last":true,"title":["Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy"],"weko_creator_id":"61","weko_shared_id":-1},"updated":"2023-06-19T08:48:16.517389+00:00"}