{"created":"2023-06-19T07:25:41.161890+00:00","id":13706,"links":{},"metadata":{"_buckets":{"deposit":"59529b43-839d-42a8-8a6c-dcead7ac8277"},"_deposit":{"created_by":78,"id":"13706","owners":[78],"pid":{"revision_id":0,"type":"depid","value":"13706"},"status":"published"},"_oai":{"id":"oai:gifu-pu.repo.nii.ac.jp:00013706","sets":["212:264:300"]},"author_link":["28960","28963","28961","28966","28964","28965","28967","28968","28959","28962"],"item_3_biblio_info_3":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"454","bibliographicPageStart":"449","bibliographicVolumeNumber":"80","bibliographic_titles":[{"bibliographic_title":"American Journal of Veterinary Research"},{"bibliographic_title":"American Journal of Veterinary Research","bibliographic_titleLang":"en"}]}]},"item_3_text_4":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_text_value":"10.2460/ajvr.80.5.449"}]},"item_3_textarea_2":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_textarea_value":"OBJECTIVE:\nTo compare ultracentrifugation, precipitation, and membrane affinity chromatography methods for isolation of extracellular vesicles (EVs) from canine plasma samples and to identify suitable reference genes for incorporation into a quantitative reverse transcription PCR assay of microRNA expression in plasma EVs of healthy dogs.\n\nANIMALS:\n6 healthy Beagles.\n\nPROCEDURES:\nPlasma samples were obtained from each dog, and EVs were isolated from 0.3 mL of these samples via ultracentrifugation, precipitation, and membrane-affinity chromatographic methods. Nanoparticle tracking analysis was performed to determine the concentration and size distribution of EVs isolated by the ultracentrifugation method. Expression levels (cycle threshold values) of 4 microRNAs (let-7a, miR-16, miR-26a, and miR-103) were then compared by means of quantitative reverse transcription PCR assay. Three statistical programs were used to identify the microRNAs most suitable for use as reference genes.\n\nRESULTS:\nResults indicated that ultracentrifugation was the most stable of all 3 methods for isolating microRNAs from 0.3 mL of plasma. Nanoparticle tracking revealed that EV samples obtained by the ultracentrifugation method contained a mean ± SD of approximately 1.59 × 1010 vesicles/mL ± 4.2 × 108 vesicles/mL. Of the 4 microRNAs in plasma EVs isolated by ultracentrifugation, miR-103 was the most stable.\n\nCONCLUSIONS AND CLINICAL RELEVANCE:\nThe ultracentrifugation method has potential as a stable method for isolating EVs from canine plasma samples with a high recovery rate, and miR-103 may provide the most stable reference gene for normalizing microRNA expression data pertaining to plasma EVs isolated by ultracentrifugation."}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of reference genes for microRNAs of extracellular vesicles isolated from plasma samples of healthy dogs by ultracentrifugation, precipitation, and membrane affinity chromatography methods","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of reference genes for microRNAs of extracellular vesicles isolated from plasma samples of healthy dogs by ultracentrifugation, precipitation, and membrane affinity chromatography methods"},{"subitem_title":"Identification of reference genes for microRNAs of extracellular vesicles isolated from plasma samples of healthy dogs by ultracentrifugation, precipitation, and membrane affinity chromatography methods","subitem_title_language":"en"}]},"item_type_id":"3","owner":"78","path":["300"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-03"},"publish_date":"2020-03-03","publish_status":"0","recid":"13706","relation_version_is_last":true,"title":["Identification of reference genes for microRNAs of extracellular vesicles isolated from plasma samples of healthy dogs by ultracentrifugation, precipitation, and membrane affinity chromatography methods"],"weko_creator_id":"78","weko_shared_id":-1},"updated":"2023-06-19T07:50:08.445662+00:00"}