{"created":"2023-06-19T07:25:49.490457+00:00","id":13926,"links":{},"metadata":{"_buckets":{"deposit":"e0e2e98b-f8f7-419e-8b66-b39a8008dd58"},"_deposit":{"created_by":89,"id":"13926","owners":[89],"pid":{"revision_id":0,"type":"depid","value":"13926"},"status":"published"},"_oai":{"id":"oai:gifu-pu.repo.nii.ac.jp:00013926","sets":["212:273:285"]},"author_link":["30816","30804","30806","30805","30808","30809","30813","30810","30803","30814","30812","30815","30807","30811"],"item_3_biblio_info_3":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageStart":"117003","bibliographicVolumeNumber":"127","bibliographic_titles":[{},{"bibliographic_title":"Environmental Health Perspectives","bibliographic_titleLang":"en"}]}]},"item_3_text_4":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_text_value":" 10.1289/EHP5039"}]},"item_3_textarea_2":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_textarea_value":"BACKGROUND:\nAtherosclerotic cardiovascular disease has become the leading cause of death worldwide, and environmental pollutants are increasingly recognized as risk factors for atherosclerosis. Liver X receptors (LXRs) play a central role in atherosclerosis; however, LXR activity of organic pollutants and associated potential risk of atherosclerosis have not yet been characterized.\n\nOBJECTIVES:\nThis study aimed to explore whether LXR-antagonistic chemicals are present in indoor house dust and, if so, to characterize this activity in relation to changes in macrophages in vitro and cardiovascular disease indicators in vivo in an atherosclerosis ApoE−/− mouse model.\n\nMETHODS:\nWe used a His-LXRα-pull-down assay and a nontarget high-resolution mass spectrometry method to screen house dust collected from Chinese homes for LXRα- and LXRβ-antagonist activity. A chemical identified in this manner was assessed for its ability to induce cholesterol efflux and foam cell formation in RAW264.7 macrophages, to down-regulate the expression of two LXR-dependent genes, ABCA1 and ABCG1, and finally to induce atherosclerotic lesions in vivo using an ApoE−/− mouse model.\n\nRESULTS:\nWe identified the flame retardants triphenyl phosphate (TPHP) and 2-ethylhexyl diphenyl phosphate (EHDPP) in house dust samples and demonstrated their ability to antagonize LXRs. The potency of TPHP was similar to that of the LXR-antagonist SR9238. TPHP could also inhibit cholesterol efflux and promote foam cell formation in RAW264.7 macrophages and mouse peritoneal macrophages and significantly promoted atherosclerotic lesion formation in the ApoE−/− mouse model.\n\nCONCLUSIONS:\nWe found LXR-antagonist chemicals in environmental samples of indoor dust from Chinese homes. One of the chemicals, TPHP, was able to promote the development of atherosclerotic lesions in the ApoE−/− mouse model. These results highlight the need to assess the LXR-antagonist activities of pollutants in future environmental management programs. "}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Screening of House Dust from Chinese Homes for Chemicals with Liver X Receptors Binding Activities and Characterization of Atherosclerotic Activity Using an            in Vitro            Macrophage Cell Line and ApoE−/− Mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Screening of House Dust from Chinese Homes for Chemicals with Liver X Receptors Binding Activities and Characterization of Atherosclerotic Activity Using an            in Vitro            Macrophage Cell Line and ApoE−/− Mice","subitem_title_language":"en"}]},"item_type_id":"3","owner":"89","path":["285"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-06"},"publish_date":"2020-03-06","publish_status":"0","recid":"13926","relation_version_is_last":true,"title":["Screening of House Dust from Chinese Homes for Chemicals with Liver X Receptors Binding Activities and Characterization of Atherosclerotic Activity Using an            in Vitro            Macrophage Cell Line and ApoE−/− Mice"],"weko_creator_id":"89","weko_shared_id":-1},"updated":"2023-06-19T07:47:56.365316+00:00"}