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Cadmium Inhibits All-Trans-Retinoic Acid-Induced Increase of Nitroblue Tetrazolium Reduction Activity and Induces Metallothionein 1G Expression in Human Acute Myelocytic Leukemia HL-60 Cells
https://gifu-pu.repo.nii.ac.jp/records/13930
https://gifu-pu.repo.nii.ac.jp/records/13930f4e0b746-9200-4068-a526-e17ed75b4c76
| Item type | 研究室原著論文(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-03-06 | |||||
| タイトル | ||||||
| タイトル | Cadmium Inhibits All-Trans-Retinoic Acid-Induced Increase of Nitroblue Tetrazolium Reduction Activity and Induces Metallothionein 1G Expression in Human Acute Myelocytic Leukemia HL-60 Cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cadmium, hematopoietic differentiation, metallothionein | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 抄録 | ||||||
| 値 | Cadmium is an environmental pollutant. Metallothioneins are cysteine-rich, low-molecular-weight proteins that are induced by cadmium, which they chelate for detoxification. In humans, the functional metallothionein isoforms are MT1A, 1B, 1E, 1F, 1G, 1H, 1M, 1X, 2A, 3, and 4. It has been reported that overexpression of MT1G inhibits all-trans-retinoic acid (ATRA)–induced hematopoietic differentiation. Here, we found that cadmium inhibited ATRA-induced nitroblue tetrazolium reduction activity, a marker of hematopoietic differentiation, in human acute myelocytic leukemia HL-60 cells. Reverse transcription – quantitative polymerase chain reaction analysis of HL-60 cells revealed the expression of the metallothionein isoforms MT1G, MT1X, and MT2A and showed that MT1G expression increased significantly after cadmium treatment. ATRA treatment significantly attenuated this cadmium-induced increase in MT1G expression; however, MT1G expression remained significantly higher than that in untreated cells. No significant changes in MT1X and MT2A expression were observed. We also found that the cadmium-induced increase in MT1G expression was independent of CpG demethylation. ATRA is a standard chemotherapy treatment for acute promyelocytic leukemia; our findings suggest that cadmium may inhibit the effect of this chemotherapy. | |||||
| 書誌情報 |
en : BPB reports 巻 3, 号 1, p. 34-38, 発行日 2020 |
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