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Dimeric dihydrodiol dehydrogenase is an efficient primate 1,5-anhydro-D-fructose reductase
https://gifu-pu.repo.nii.ac.jp/records/14277
https://gifu-pu.repo.nii.ac.jp/records/14277dfdcbb1e-15cf-403f-977e-c138b6314acd
| Item type | 研究室原著論文(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-03-04 | |||||
| タイトル | ||||||
| タイトル | Dimeric dihydrodiol dehydrogenase is an efficient primate 1,5-anhydro-D-fructose reductase | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | 1,5-Anhydro-D-fructose reductase; 1,5-Anhydro-D-glucitol; AKR1A1; D-xylose dehydrogenase; DHDH; Dicarbonyl/L-xylulose reductase. | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 抄録 | ||||||
| 値 | 1,5-Anhydro-D-fructose (AF), a metabolite of the anhydrofructose pathway of glycogen metabolism, has recently been shown to react with intracellular proteins and form advanced glycation end-products. The reactive AF is metabolized to non-reactive 1,5-anhydro-D-glucitol by AF reductase in animal tissues and human cells. Pig and mouse AF reductases were characterized, but primate AF reductase remains unknown. Here, we examined the AF-reducing activity of eleven primate NADPH-dependent reductases with broad substrate specificity for carbonyl compounds. AF was reduced by monkey dimeric dihydrodiol dehydrogenase (DHDH), human aldehyde reductase (AKR1A1) and human dicarbonyl/L-xylulose reductase (DCXR). DHDH showed the lowest KM (21 μM) for AF, and its kcat/KM value (1208 s-1mM-1) was much higher than those of AKR1A1 (1.3 s-1mM-1), DCXR (1.1 s-1mM-1) and the pig and mouse AF reductases. AF is a novel substrate with higher affinity and catalytic efficiency than known substrates of DHDH. Docking simulation study suggested that Lys156 in the substrate-binding site of DHDH contributes to the high affinity for AF. Gene database searches identified DHDH homologues (with >95% amino acid sequence identity) in humans and apes. Thus, DHDH acts as an efficient AF reductase in primates. | |||||
| 書誌情報 |
en : Biochemical and Biophysical Research Communications 巻 526, 号 3, p. 728-732, 発行日 2020-06-04 |
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| DOI | ||||||
| 値 | 10.1016/j.bbrc.2020.03.176 | |||||
