{"created":"2023-06-19T07:26:03.846183+00:00","id":14308,"links":{},"metadata":{"_buckets":{"deposit":"3b3059e9-3b85-4a87-921a-f838c64e2bf8"},"_deposit":{"created_by":124,"id":"14308","owners":[124],"pid":{"revision_id":0,"type":"depid","value":"14308"},"status":"published"},"_oai":{"id":"oai:gifu-pu.repo.nii.ac.jp:00014308","sets":["212:260:312"]},"author_link":["33192","33193","33194","33197","33196","33195"],"item_3_biblio_info_3":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"122","bibliographicPageStart":"115","bibliographicVolumeNumber":"41","bibliographic_titles":[{"bibliographic_title":"CHROMATOGRAPHY"},{"bibliographic_title":"CHROMATOGRAPHY","bibliographic_titleLang":"en"}]}]},"item_3_textarea_2":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_textarea_value":"This report presents two fluorescence labeling methods for therapeutic monoclonal antibody, bevacizumab, to increase its detection sensitivity for fluorescence detection. One method is high-temperature reversed-phase LC (HT-RPLC) following post-column fluorogenic derivatization using o-phthalaldehyde with thiol. Another method is pre-column derivatization using Zenon Alexa Fluor 488 protein-tag following size-exclusion chromatography (SEC). The calibration curves of bevacizumab were 1–50 μg/mL (post-column method) and 0.1–10 μg/mL (pre-column method). Both methods showed good correlation coefficients (r2 > 0.991). The LOD and the LOQ of bevacizumab were, respectively, 0.13 and 0.43 μg/mL (post-column method) and 0.03 and 0.1 μg/mL (pre-column method). The sensitivities were about 2 and 10 times higher than that of native fluorescence detection. The proposed methods were applied to bevacizumab spiked human plasma samples. The bevacizumab in plasma samples was purified selectively with immunoaffinity beads and detected as a single peak using HT-RPLC or SEC with fluorescence detection."}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bevacizumab","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluorescence derivatization","subitem_subject_scheme":"Other"},{"subitem_subject":"High-temperature reversed-phase liquid chromatography","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunoaffinity purification","subitem_subject_scheme":"Other"},{"subitem_subject":"Bioanalysis","subitem_subject_scheme":"Other"},{"subitem_subject":"Bevacizumab","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluorescence derivatization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"High-temperature reversed-phase liquid chromatography","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunoaffinity purification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bioanalysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Fluorescence Bioanalysis of Bevacizumab Using Pre-Column and Post-Column Derivatization – Liquid Chromatography After Immunoaffinity Magnetic Purification","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Fluorescence Bioanalysis of Bevacizumab Using Pre-Column and Post-Column Derivatization – Liquid Chromatography After Immunoaffinity Magnetic Purification"},{"subitem_title":"Fluorescence Bioanalysis of Bevacizumab Using Pre-Column and Post-Column Derivatization – Liquid Chromatography After Immunoaffinity Magnetic Purification","subitem_title_language":"en"}]},"item_type_id":"3","owner":"124","path":["312"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-03-08"},"publish_date":"2021-03-08","publish_status":"0","recid":"14308","relation_version_is_last":true,"title":["Fluorescence Bioanalysis of Bevacizumab Using Pre-Column and Post-Column Derivatization – Liquid Chromatography After Immunoaffinity Magnetic Purification"],"weko_creator_id":"124","weko_shared_id":-1},"updated":"2023-06-19T07:44:10.007640+00:00"}