Molecular Imaging of Labile Heme in Living Cells Using a Small Molecule Fluorescent Probe

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Molecular Imaging of Labile Heme in Living Cells Using a Small Molecule Fluorescent Probe

https://gifu-pu.repo.nii.ac.jp/records/14563

Item type		研究室原著論文(1)
公開日		2022-03-07
タイトル
	タイトル	Molecular Imaging of Labile Heme in Living Cells Using a Small Molecule Fluorescent Probe
タイトル
	タイトル	Molecular Imaging of Labile Heme in Living Cells Using a Small Molecule Fluorescent Probe
	言語	en
言語
	言語	eng
資源タイプ
	資源タイプ識別子	http://purl.org/coar/resource_type/c_6501
	資源タイプ	journal article
アクセス権
	アクセス権	metadata only access
	アクセス権URI	http://purl.org/coar/access_right/c_14cb
抄録
	値	Labile heme (LH) is a complex of Fe(II) and protoporphyrin IX, an essential signaling molecule in various biological systems. Most of the subcellular dynamics of LH remain unclear because of the lack of efficient chemical tools for detecting LH in cells. Here, we report an activity-based fluorescence probe that can monitor the fluctuations of LH in biological events. H-FluNox is a selective fluorescent probe that senses LH using biomimetic N-oxide deoxygenation to trigger fluorescence. The selectivity of H-FluNox to LH is >100-fold against Fe(II), enabling the discrimination of LH from the labile Fe(II) pool in living cells. The probe can detect the acute release of LH upon NO stimulation and the accumulation of LH by inhibiting the heme exporter. In addition, imaging studies using the probe revealed a partial heme-export activity of the ATP-binding cassette subfamily G member 2 (ABCG2), potential LH pooling ability of G-quadruplex, and involvement of LH in ferroptosis. The successful use of H-FluNox in identifying fluctuations of LH in living cells offers opportunities for studying the physiology and pathophysiology of LH in living systems.
書誌情報		en : Journal of the American Chemical Society 発行日 2022