@article{oai:gifu-pu.repo.nii.ac.jp:00006852,
 author = {杉浦, 衛 and 加納, 邦雄 and 清水, 浩 and スギウラ, マモル and カノウ, クニオ and シミズ, ヒロシ and Sugiura, Mamoru and Kano, Kunio and Shimizu, Hiroshi},
 journal = {岐阜藥科大學紀要, The annual proceedings of Gifu College of Pharmacy},
 month = {Nov},
 note = {P(論文), An uricase was extracted by freezing, thawing and autolysis from Candida utilis grown on a medium contained of uric acid . The uricase was purified by ammonium sulfate fractionation (0.3-0.5 saturation), Sephadex G-100 and DEAE-cellulose column chromatography. The enzyme was purified about 80-fold by this procedure from original extracts. The enzymatic properties of the purified enzyme was studied . Optimum pH was 8.0,stable pH range was from 7.0 to 11.0 and optimum temperature was 35℃. The activity was inhibited by Cu^<2+>, Mn^<2+>, CN-and PCMB, while the activity was activated by glutathion. The lyophilized enzyme was stable at room temperature for a long time.},
 pages = {22--29},
 title = {<原報>酵母ウリカーゼに関する研究(1)酵母ラリカーゼの部分精製について(酵素剤の研究第56報)},
 volume = {19},
 year = {1969}
}