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  1. 教員研究業績
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  3. 原著論文

Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy

https://gifu-pu.repo.nii.ac.jp/records/13457
https://gifu-pu.repo.nii.ac.jp/records/13457
259b9ce0-61af-4303-8b94-a2938c1e3942
Item type 会議発表論文 / Conference Paper(1)
公開日 2019-03-04
タイトル
タイトル Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_5794
資源タイプ conference paper
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Shi, Lei

× Shi, Lei

WEKO 21801

Shi, Lei

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Ito, Fumiya

× Ito, Fumiya

WEKO 21802

Ito, Fumiya

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Wang, Yue

× Wang, Yue

WEKO 21803

Wang, Yue

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Okazaki, Yasumasa

× Okazaki, Yasumasa

WEKO 21804

Okazaki, Yasumasa

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Tanaka, Hiromasa

× Tanaka, Hiromasa

WEKO 21805

Tanaka, Hiromasa

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Mizuno, Masaaki

× Mizuno, Masaaki

WEKO 21806

Mizuno, Masaaki

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Hori, Masaru

× Hori, Masaru

WEKO 21807

Hori, Masaru

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Hirayama, Tasuku

× Hirayama, Tasuku

WEKO 21808

Hirayama, Tasuku

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Nagasawa, Hideko

× Nagasawa, Hideko

WEKO 21809

Nagasawa, Hideko

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Richardson, Des R.

× Richardson, Des R.

WEKO 21810

Richardson, Des R.

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Toyokuni, Shinya

× Toyokuni, Shinya

WEKO 21811

Toyokuni, Shinya

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内容記述タイプ Abstract
内容記述 Non-thermal plasma (NTP) is a potential new therapeutic modality for cancer. However, its mechanism of action remains unclear. Herein, we studied the effect of NTP on mesothelioma cells and fibroblasts to understand its anti-proliferative efficacy. Interestingly, NTP demonstrated greater selective anti-proliferative activity against mesothelioma cells relative to fibroblasts than cisplatin, which is used for mesothelioma treatment. The anti-proliferative effect of NTP was enhanced by pre-incubation with the cellular iron donor, ferric ammonium citrate (FAC), and inhibited by iron chelation using desferrioxamine (DFO). Three oxidative stress probes (CM-H2DCFDA, MitoSOX and C11-BODIPY) demonstrated reactive oxygen species (ROS) generation by NTP, which was inhibited by DFO. Moreover, NTP decreased transferrin receptor-1 and increased ferritin-H and -L chain expression that was correlated with decreased iron-regulatory protein expression and RNA-binding activity. This regulation was potentially due to increased intracellular iron in lysosomes, which was demonstrated via the Fe(II)-selective probe, HMRhoNox-M, and was consistent with autophagic-induction. Immunofluorescence using LysoTracker and Pepstatin A probes demonstrated increased cellular lysosome content, which was confirmed by elevated LAMP1 expression. The enhanced lysosomal biogenesis after NTP could be due to the observed increase in fluid-phase endocytosis and early endosome formation. These results suggest NTP acts as a stressor, which results in increased endocytosis, lysosome content and autophagy. In fact, NTP rapidly increased autophagosome formation, as judged by increased LC3B-II expression, which co-localized with LAMP1, indicating autophagolysosome formation. Autophagic-induction by NTP was confirmed using electron microscopy. In summary, NTP acts as a cellular stressor to rapidly induce fluid-phase endocytosis, lysosome biogenesis and autophagy.
書誌情報 en : Free Radical Biology and Medicine

巻 108, p. 904-917, 発行日 2017
ISSN
収録物識別子タイプ ISSN
収録物識別子 08915849
DOI
識別子タイプ DOI
関連識別子 10.1016/j.freeradbiomed.2017.04.368
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