{"_buckets": {"deposit": "8113eb7a-0ee5-4fa9-a92f-99cbb697f7f9"}, "_deposit": {"created_by": 61, "id": "13455", "owners": [61], "pid": {"revision_id": 0, "type": "depid", "value": "13455"}, "status": "published"}, "_oai": {"id": "oai:gifu-pu.repo.nii.ac.jp:00013455", "sets": ["259", "315"]}, "author_link": ["21784", "21785", "21788", "21786", "21783", "21789", "21787"], "item_10003_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "290", "bibliographicPageStart": "279", "bibliographicVolumeNumber": "34", "bibliographic_titles": [{}, {"bibliographic_title": "Cell Biology and Toxicology", "bibliographic_titleLang": "en"}]}]}, "item_10003_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We recently demonstrated the cytotoxic action of a novel phenformin derivative, 2-(2-chlorophenyl)ethylbiguanide (2-Cl-Phen), on HT-29 cells under a serum- and glucose-deprived condition. In that study, we showed that the ATF6 arm of the ER stress pathway and c-Myc expression were downregulated 12 h after the treatment with 2-Cl-Phen. Through characterization of intracellular events at the early phase of the 2-Cl-Phen treatment before noticeable morphological changes, we found rapid fluctuations in the c-Myc and ATF4 proteins but not in their mRNAs in 2-Cl-Phen-treated HT-29 cells under the serum- and glucose-deprived condition. The 2-Cl-Phen-mediated downregulation of ATF4 protein was not paralleled by the phosphorylation status of PERK and eIF2α. Reduction of c-Myc expression by 2-Cl-Phen was more profound than that of ATF4 expression, and phosphorylated c-Myc was downregulated within 2 h. Pharmacological studies on the expression of c-Myc and ATF4 proteins showed that this decrease was mediated through proteasomal degradation but not by autophagy. Interestingly, treatment with lithium chloride, which is a well-known inhibitor of GSK3β, partially recovered the expression of ATF4 protein, but its effect on the level of total c-Myc protein was negligible. Treatment with 2-Cl-Phen increased the expression of phosphorylated AMPK, but Compound C, an AMPK inhibitor, did not influence the expression of c-Myc protein in HT-29 cells. Finally, we observed that 2-Cl-Phen partially attenuated the gene expression of integrin subunit α1 (ITGA1), a downstream target of c-Myc. Taken together, these results show that 2-Cl-Phen rapidly downregulated the expression of c-Myc in addition to ER stress responses in a post-translational manner. Further elucidation and improvement of this multi-target-directed compound will provide new insights for developing therapeutic strategies against cancer.", "subitem_description_type": "Abstract"}]}, "item_10003_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1007/s10565-017-9410-0", "subitem_relation_type_select": "DOI"}}]}, "item_10003_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0742-2091", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Oh-hashi, Kentaro"}], "nameIdentifiers": [{"nameIdentifier": "21783", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Shiori"}], "nameIdentifiers": [{"nameIdentifier": "21784", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakai, Takayuki"}], "nameIdentifiers": [{"nameIdentifier": "21785", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nomura, Yuki"}], "nameIdentifiers": [{"nameIdentifier": "21786", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okuda, Kensuke"}], "nameIdentifiers": [{"nameIdentifier": "21787", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagasawa, Hideko"}], "nameIdentifiers": [{"nameIdentifier": "21788", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirata, Yoko"}], "nameIdentifiers": [{"nameIdentifier": "21789", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference paper", "resourceuri": "http://purl.org/coar/resource_type/c_5794"}]}, "item_title": "Elucidating the rapid action of 2-(2-chlorophenyl)ethylbiguanide on HT-29 cells under a serum- and glucose-deprived condition", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Elucidating the rapid action of 2-(2-chlorophenyl)ethylbiguanide on HT-29 cells under a serum- and glucose-deprived condition", "subitem_title_language": "en"}]}, "item_type_id": "10003", "owner": "61", "path": ["259", "315"], "permalink_uri": "https://gifu-pu.repo.nii.ac.jp/records/13455", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-03-04"}, "publish_date": "2019-03-04", "publish_status": "0", "recid": "13455", "relation": {}, "relation_version_is_last": true, "title": ["Elucidating the rapid action of 2-(2-chlorophenyl)ethylbiguanide on HT-29 cells under a serum- and glucose-deprived condition"], "weko_shared_id": -1}