{"_buckets": {"deposit": "3c8cea30-805a-49a6-9f89-c439410875eb"}, "_deposit": {"created_by": 61, "id": "13456", "owners": [61], "pid": {"revision_id": 0, "type": "depid", "value": "13456"}, "status": "published"}, "_oai": {"id": "oai:gifu-pu.repo.nii.ac.jp:00013456", "sets": ["259", "315"]}, "author_link": ["21798", "21800", "21795", "21792", "21790", "21799", "21791", "21793", "21796", "21794", "21797"], "item_10003_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "182", "bibliographicPageStart": "176", "bibliographicVolumeNumber": "61", "bibliographic_titles": [{}, {"bibliographic_title": "Journal of Clinical Biochemistry and Nutrition", "bibliographic_titleLang": "en"}]}]}, "item_10003_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Congenital diaphragmatic hernia (CDH) is a life-threatening neonatal disease that leads to lung hypoplasia and pulmonary hypertension. We recently found that maternal prenatal administration of Saireito (TJ-114) ameliorates fetal CDH in a nitrofen-induced rat model. Here, we studied the role of iron and oxidative stress in neonates of this model and in lung fibroblasts IMR90-SV in association with nitrofen and Saireito. We observed increased immunostaining of 8-hydroxy-2\u0027-deoxyguanosine in the lungs of neonates with CDH, which was ameliorated by maternal Saireito intake. Pulmonary transferrin receptor expression was significantly decreased in both CDH and CDH after Saireito in comparison to normal controls, indicating functional lung immaturity, whereas catalytic Fe(II) and pulmonary DMT1/ferroportin expression remained constant among the three groups. Saireito revealed a dose-dependent scavenging capacity with electron spin resonance spin trapping in vitro against hydroxyl radicals but not against superoxide. Finally, nitrofen revealed dose-dependent cytotoxicity to IMR90-SV cells, accompanied by an increase in oxidative stress, as seen by 5(6)-chloromethyl-2\u0027,7\u0027-dichlorodihydrofluorescein diacetate and catalytic Fe(II). Saireito ameliorated all of these in IMR90-SV cells. In conclusion, catalytic Fe(II)-dependent oxidative stress by nitrofen may be the pathogenic cause of CDH, and the antioxidative activity of Saireito is at least partially responsible for improving nitrofen-induced CDH.", "subitem_description_type": "Abstract"}]}, "item_10003_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.3164/jcbn.17-17", "subitem_relation_type_select": "DOI"}}]}, "item_10003_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0912-0009", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hirako, Shima"}], "nameIdentifiers": [{"nameIdentifier": "21790", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuda, Hiroyuki"}], "nameIdentifiers": [{"nameIdentifier": "21791", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, Fumiya"}], "nameIdentifiers": [{"nameIdentifier": "21792", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okazaki, Yasumasa"}], "nameIdentifiers": [{"nameIdentifier": "21793", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirayama, Tasuku"}], "nameIdentifiers": [{"nameIdentifier": "21794", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagasawa, Hideko"}], "nameIdentifiers": [{"nameIdentifier": "21795", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakano, Tomoko"}], "nameIdentifiers": [{"nameIdentifier": "21796", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imai, Kenji"}], "nameIdentifiers": [{"nameIdentifier": "21797", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kotani, Tomomi"}], "nameIdentifiers": [{"nameIdentifier": "21798", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kikkawa, Fumitaka"}], "nameIdentifiers": [{"nameIdentifier": "21799", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Toyokuni, Shinya"}], "nameIdentifiers": [{"nameIdentifier": "21800", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference paper", "resourceuri": "http://purl.org/coar/resource_type/c_5794"}]}, "item_title": "Role of catalytic iron and oxidative stress in nitrofen-induced congenital diaphragmatic hernia and its amelioration by Saireito (TJ-114)", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Role of catalytic iron and oxidative stress in nitrofen-induced congenital diaphragmatic hernia and its amelioration by Saireito (TJ-114)", "subitem_title_language": "en"}]}, "item_type_id": "10003", "owner": "61", "path": ["259", "315"], "permalink_uri": "https://gifu-pu.repo.nii.ac.jp/records/13456", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-03-04"}, "publish_date": "2019-03-04", "publish_status": "0", "recid": "13456", "relation": {}, "relation_version_is_last": true, "title": ["Role of catalytic iron and oxidative stress in nitrofen-induced congenital diaphragmatic hernia and its amelioration by Saireito (TJ-114)"], "weko_shared_id": -1}