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Preparation of N2-Ethyl-2′-deoxyguanosine-d4 as an Internal Standard for the Electrospray Ionization–Tandem Mass Spectrometric Determination of DNA Damage by Acetaldehyde
https://gifu-pu.repo.nii.ac.jp/records/14517
https://gifu-pu.repo.nii.ac.jp/records/14517fcb77049-21ef-437d-a8ef-172de0ac679a
| Item type | 研究室原著論文(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-03-06 | |||||
| タイトル | ||||||
| タイトル | Preparation of N2-Ethyl-2′-deoxyguanosine-d4 as an Internal Standard for the Electrospray Ionization–Tandem Mass Spectrometric Determination of DNA Damage by Acetaldehyde | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | N2-Ethyl-2′-deoxyguanosine-d4 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | isotopic internal standard | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | ESI-MS/MS | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | acetaldehyde-d4 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | D2O | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cancer risk marker | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 抄録 | ||||||
| 値 | The deuteration of N2-ethyl-2′-deoxyguanosine (Et-dG), which is a DNA adduct generated from acetaldehyde, was studied by the addition reaction of acetaldehyde-d4 to 2′-deoxyguanosine (dG) in deuterium oxide (D2O), with the aim to obtain an isotope internal standard for the liquid chromatography/tandem mass spectrometry (LC/MS/MS) quantitation of Et-dG. The replacement of the dG C-8 hydrogen atom by a deuteron atom took place at 50°C in D2O and afforded a mixture of Et-dG-d4 and Et-dG-d5. Et-dG-d4, which was stable in aqueous solutions, was prepared by incubating the mixture in H2O at 60°C for 48 h. The calibration curve was obtained by multiple reaction monitoring (MRM) measurements using a hydrophilic interaction chromatography–electrospray ionization–tandem mass spectrometric (HILIC/ESI-MS/MS) system between the Et-dG concentration, ranging from 1.0 × 10−10 to 4.0 × 10−9 M in the sample solutions, and the relative peak areas of Et-dG (m/z: 296.1 → 180.1) to the value of Et-dG-d4 (m/z: 300.2 → 184.2), with an internal standard showing good linearity (R2 = 0.995, n = 5). | |||||
| 書誌情報 |
en : Analytical Sciences 巻 36, 号 7, p. 877-880, 発行日 2020 |
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| DOI | ||||||
| 値 | 10.2116/analsci.19N034 | |||||
