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極性化合物捕集用メタクリレート系逆相型固相抽出剤の調製と特性評価
https://gifu-pu.repo.nii.ac.jp/records/14527
https://gifu-pu.repo.nii.ac.jp/records/1452766d3aa1d-a082-4276-b94d-c43df0e2d010
Item type | 研究室原著論文(1) | |||||
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公開日 | 2022-03-06 | |||||
タイトル | ||||||
タイトル | 極性化合物捕集用メタクリレート系逆相型固相抽出剤の調製と特性評価 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Preparation and Evaluation of Methacrylate-based Reversed-phase Sorbents for Solid-phase Extraction of Polar Compounds | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
抄録 | ||||||
値 | The concept of comprehensive DNA adducts analysis (called DNA adductomics) has recently been attracting attention, and the development of a highly sensitive and accurate quantitative method for DNA adductomics has been desired. In our previous research on LC-ESI-MS/MS, hydrophilic interaction chromatography (HILIC) was found to be effective for improving the sensitivity concerning the quantitation of DNA adducts. Although HILIC-ESI-MS/MS is useful for the quantitation of DNA adducts, the improvement of HILIC separation for DNA adductomics must be still needed. In this paper, we describe the improvement of HILIC separations for DNA adductomics by investigating the composition of mobile phases and the commercially available HILIC columns with four difference polar groups. While the elution order for four 2'-deoxynucleosides and the two DNA adducts was not drastically changed by varying the HILIC columns, the separation of each dN and DNA adducts was improved by adding alcohols and by varying the alcoholic concentration. | |||||
書誌情報 |
en : BUNSEKI KAGAKU 巻 69, 号 1.2, p. 25-30, 発行日 2020 |