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<原報>酵母ウリカーゼに関する研究(1)酵母ラリカーゼの部分精製について(酵素剤の研究第56報)
https://gifu-pu.repo.nii.ac.jp/records/6852
https://gifu-pu.repo.nii.ac.jp/records/68522d2d59c4-90cc-4fa1-bb3e-1f1d9032f815
名前 / ファイル | ライセンス | アクション |
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KJ00000071060 (493.5 kB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 1969-11-30 | |||||
タイトル | ||||||
タイトル | <原報>酵母ウリカーゼに関する研究(1)酵母ラリカーゼの部分精製について(酵素剤の研究第56報) | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | <Originals>Studies on Yeast Uricase. 1 Partially purification of Yeast Uricase (Studies on Enzymes LVI) | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
論文名よみ | ||||||
その他のタイトル | 〓 | |||||
著者名(日) |
杉浦, 衛
× 杉浦, 衛× 加納, 邦雄× 清水, 浩 |
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著者名よみ |
スギウラ, マモル
× スギウラ, マモル× カノウ, クニオ× シミズ, ヒロシ |
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著者名(英) |
Sugiura, Mamoru
× Sugiura, Mamoru× Kano, Kunio× Shimizu, Hiroshi |
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著者所属(日) | ||||||
値 | /小野薬品中央研究所/小野薬品中央研究所 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | An uricase was extracted by freezing, thawing and autolysis from Candida utilis grown on a medium contained of uric acid . The uricase was purified by ammonium sulfate fractionation (0.3-0.5 saturation), Sephadex G-100 and DEAE-cellulose column chromatography. The enzyme was purified about 80-fold by this procedure from original extracts. The enzymatic properties of the purified enzyme was studied . Optimum pH was 8.0,stable pH range was from 7.0 to 11.0 and optimum temperature was 35℃. The activity was inhibited by Cu^<2+>, Mn^<2+>, CN-and PCMB, while the activity was activated by glutathion. The lyophilized enzyme was stable at room temperature for a long time. | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00053514 | |||||
書誌情報 |
岐阜藥科大學紀要 en : The annual proceedings of Gifu College of Pharmacy 巻 19, p. 22-29, 発行日 1969-11-30 |